Table 1.
Activity against the promastigotes and intracellular amastigotes of Leishmania amazonensis, cytotoxicity in BALB/c peritoneal macrophages, and selectivity index of oil and limonoid-rich fractions of Carapa guianensis seed oil.
| Compounds | IC50 (μg/mL) promastigote | CC50 (μg/mL) peritoneal macrophage | IC50 (μg/mL) intracellular amastigote | IS |
|---|---|---|---|---|
| C. guianensis seed oil | >500 | >1000 | – | – |
| LF1 | >500 | >1000 | – | – |
| LF2 | >500 | 489.4 ± 0.173 | – | – |
| LF3 | 10.53 ± 0.050 | 78.55 ± 1.406 | 27.31 ± 0.091 | 2.87 |
| LF4 | 25.30 ± 0.057 | 139.0 ± 1.523 | 78.42 ± 0.086 | 1.77 |
| LF5 | 56.90 ± 0.043 | 607.70 ± 1.217 | 352.2 ± 0.145 | 1.72 |
| LF6 | >500 | >1000 | – | – |
| Amphotericin B | 1.37±0.124 | 6.26±0.286 | 0.2 ± 0.180 | 31.3 |
IC50: inhibitory concentration of 50% parasites. CC50: cytotoxicity concentration of 50% cells. SI: selectivity index calculated from the ratio of CC50 versus the IC50 for intracellular amastigotes. LF: limonoid-rich fraction of C. guianensis seed oil. Data represent mean ± standard deviation of three experiments performed at least in triplicate.