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VP5 EE>AA mutant forms intranuclear clusters of capsid proteins. Vero cells were infected at an MOI of 5. (A) Live-cell imaging at 8.5 hpi using derivatives of HSV-1 encoding mCherry-VP24 and either VP5 EE>AA or Δ6F10. (B) Fixed-cell immunofluorescence time course of cells infected with unmodified HSV-1 (WT), EE>AA mutant, and VP5 repair (REP). Cells were fixed at the indicated time postinfection and stained with an αVP5 antibody. Scale bars, 10 μm.
