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VP5 EE>AA focus composition is consistent with that of procapsids. Vero cells infected with HSV-1 encoding WT or EE>AA VP5 at an MOI of 5 were fixed at 8 hpi and processed for immunofluorescence using αVP5 and αVP26 antibodies. In some instances, the viruses encoded mCherry-VP24 or pUL25/mCherry fusions, as indicated. Scale bar, 10 μm.
