TABLE 2.
Primers used for RED-mediated recombination unique to this study
| Strain | Template | Primer paira |
|---|---|---|
| HSVF-GS5798 | pEP-KanS2 | 5′ TACGCCACCCTGCAGAACATGGTGGTCCCGGCGATCGCCCCCGGCGCGGAGTGCCCCAGCGACAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GTGCAGGGGGTGCGCGGGGTCCGTCACGGGGTCGCTGGGGCACTCCGCGCCGGGGGCGATCGCCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS5799 | pEP-KanS2 | 5′ GTGACGGACCCCGCGCACCCCCTGCACCCGGCCAATCTGGTGGCCGCGACCGTGGCCGCCATGTTTCACAACAGGATGACGACGATAAGTAGGG 3′ |
| 5′ CATGGCGGGCCCGTCCACTACCACGCGCCCGTTGTGAAACATGGCGGCCACGGTCGCGGCCACCAGATTGGCCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS5801 | pEP-KanS2 | 5′ CCCCGGCGAGGAGTGCCCCAGCGACCCCGTGACGGACCCCGCGGCGCCGCTAGCCCCGGCCAATCTGGTGAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GTTGTGAAACATGGCGTTGACCGTGTTGGCCACCAGATTGGCCGGGGCTAGCGGCGCCGCGGGGTCCGTCACCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS5905 | pEP-KanS2 | 5′ CCAGCGACCCCGTGACGGACCCCGCGGCGCCGCTAGCCCCGGCCGCTGCAGCGGCAAACGCGGTCAACGCCATGTTTAGGATGACGACGATAAGTAGGG 3′ |
| 5′ CCGTCCACTACCACGCGCCCGTTGTGAAACATGGCGTTGACCGCGTTTGCCGCTGCAGCGGCCGGGGCTAGCGGCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS6276 | pEP-KanS2 | 5′ CCCCGGCGAGGAGTGCCCCAGCGACCCCGTGACGGACCCCGGAGGCAGCGGAGGATCCACGGTCAACGCCATGAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GTCCACTACCACGCGCCCGTTGTGAAACATGGCGTTGACCGTGGATCCTCCGCTGCCTCCGGGGTCCGTCACGGGCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS6197 | pEP-KanS2 | 5′ CACCCTGCAGAACATGGTGGTCCCGGAGATCGCGCCCGGGGCGGAGTGCCCCAGCGACAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GGGGTGCGCGGGGTCCGTCACGGGGTCGCTGGGGCACTCCGCCCCGGGCGCGATCTCCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS6434 | pEP-KanS2 | 5′ CGCTTCGACCGCGTATACGCCACCCTGCAGAACATGGTGGTCCCGGCGATCGCCCCCGGCGAGAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GGGGTCCGTCACGGGGTCGCTGGGGCACTCCTCGCCGGGGGCGATCGCCGGGACCACCATGTTCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS6462 | pEP-KanS2 | 5′ TACGCCACCCTGCAGAACATGGTGGTCCCGGAGATCGCGCCCGGGGAGGAGTGCCCCAGCGACAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GTGCAGGGGGTGCGCGGGGTCCGTCACGGGGTCGCTGGGGCACTCCTCCCCGGGCGCGATCTCCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS5748 | pEP-mCherry-in | 5′ GACCGTTGCGCCTTTTTTTTTTTTCGTCCACCAAAGTCTCTGTGGGTGCGCGCATGGTGAGCAAGGGCGAGGAG 3′ |
| 5′ CAGGGGCTCCTCCATCCGGTCTCCCGGGGCATCGGCTGCACCACCTCCGCCACCCTTGTACAGCTCGTCCATGC 3′ | ||
| HSVF-GS6460 | pEP-mCherry-in | 5′ GACCGTTGCGCCTTTTTTTTTTTTCGTCCACCAAAGTCTCTGTGGGTGCGCGCATGGTGAGCAAGGGCGAGGAG 3′ |
| 5′ CAGGGGCTCCTCCATCCGGTCTCCCGGGGCATCGGCTGCACCACCTCCGCCACCCTTGTACAGCTCGTCCATGC 3′ | ||
| HSVF-GS6493 | pEP-mCherry-in | 5′ GACCGTTGCGCCTTTTTTTTTTTTCGTCCACCAAAGTCTCTGTGGGTGCGCGCATGGTGAGCAAGGGCGAGGAG 3′ |
| 5′ CAGGGGCTCCTCCATCCGGTCTCCCGGGGCATCGGCTGCACCACCTCCGCCACCCTTGTACAGCTCGTCCATGC 3′ | ||
| HSVF-GS5797 | pEP-KanS2 | 5′ TACGCCACCCTGCAGAACATGGTGGTCCCGGCGATCGCCCCCGGCGCGGAGTGCCCCAGCGACAGGATGACGACGATAAGTAGGG 3′ |
| 5′ GTGCAGGGGGTGCGCGGGGTCCGTCACGGGGTCGCTGGGGCACTCCGCGCCGGGGGCGATCGCCAACCAATTAACCAATTCTGATTAG 3′ | ||
| HSVF-GS6199 | pEP-KanS2 | 5′ CCCCATATCGCTTCCCGACCTCCGGTCCCGATGGCCGTCCCGCAATGAGGCCCAGGGAGTAGGATGACGACGATAAGTAGGG 3′ |
| 5′ TCTTTTATTGATTAAAACACCCCAGAAGGAACTCCCTGGGCCTCATTGCGGGACGGCCATCAACCAATTAACCAATTCTGATTAG 3′ |
a
Sequences in bold share homology to the template plasmid.