TABLE 1.
Infection of cats with multiple viral pathogensa
| Virus and/or parameter | Assay | No. of cats positive/total | Total % positive | Median copy load (copies/million cells) |
|---|---|---|---|---|
| FeLV p27 antigen | ELISA SNAP test | 32/65 | 49 | NA |
| FeLV provirus | qPCR | 43/65 | 66 | 8.4 × 106 |
| FeLV viremia | qPCR | 34/65 | 52 | 1.9 × 108 copies/ml of plasma |
| FeLV-B | Conventional PCR | 22/32 | 68 | NA |
| enFeLV copy no. | qPCR | 65/65 | 100 | 34.5 copies/cell |
| FFV provirus | qPCR | 39/65 | 60 | 3.3 × 103 |
| FCoV serology | ELISA | 20/65 | 30 | NA |
| FCoV | qPCR | 1/65 | 1 | NA |
| FcaGHV-1 | qPCR | 8/65 | 12 | 1.9 × 103 |
| FIV antibody | ELISA SNAP test | 0/65 | 0 | NA |
a
Plasma and serum were used to detect FeLV p27 antigen and FIV antibody by ELISA SNAP test and in FCoV serology testing. RNA was extracted from plasma to test for FeLV viremia and FCoV by qPCR. All other tests utilized DNA extracted from PBMCs or whole blood. NA, not available.