FIG 3.

Expression of the 135 protein inhibits NF-κB signaling pathway activation. HEK293T cells were cotransfected with p135, pN1L, and pCAGGS together with pNFκB-luc and pTK-Renilla. At 24 h, cells were treated with 100 ng/ml of TNF-α (AI) or IL-1β (BI) or left untreated for 8 h. The cell lysates were prepared for assays of NF-κB-inducible luciferase activity (AI and BI), and mRNAs were extracted for determination of TNF-α and IL-1β mRNA levels by qPCR (AIII and BIII). (AII and BII) Expression of the GTPV 135 protein and the VACV N1L protein was measured by Western blotting. Data are representative of independent experiments performed in triplicate. The significance of differences was determined by the t test. *, P < 0.05; **, P < 0.01.