FIG 3.

LMP1 outgrowth is attributable to restoration of growth potential following reattachment to extracellular matrix. (A) Anoikis-induced cell cycle arrest was assessed by resazurin assay and analyzed after 12, 24, and 48 h on poly-HEMA-coated wells. The RFU and standard deviations were determined from 3 biological replicates. (B) Immunoblot analysis of cell cycle proteins p130, p27, and PCNA after 48 h incubation of HK1 stable cells on tissue culture-treated dishes (TC dish) or poly-HEMA-coated dishes. HSC70 was used as a loading control. (C) Outgrowth of HK1 pBabe and LMP1 cells following induction of anoikis was examined by resazurin assay at 1 and 6 days postreseeding after 48 h incubation on poly-HEMA-coated dishes. (D) Crystal violet staining of recovered HK1 stable cell lines 1 and 8 days following induction of anoikis. Fibronectin, a component of extracellular matrix, was included to facilitate attachment of HK1 pBabe cells.