FIG 4.

Activation of early viral promoters and MLP by wt and DM E1A. (a) RT-PCR using primers spanning the major E1A splice sites with total cell RNA from HBTECs coinfected with the wt E1A-expressing virus at an MOI of 5 and dl312 at an MOI of 95 or infected with the DM or 183-188A vectors alone at an MOI of 100. The lengths of the pre-mRNA, 12S, and 13S transcripts are indicated. Only the region of the gel with visible bands is shown. (b) Western blot showing wt and DM large E1A protein levels at 12 and 18 h p.i. HBTEC cells were coinfected with the wt E1A-expressing virus at an MOI of 5 and dl312 at an MOI of 95 or infected with the DM virus alone at an MOI of 100. Ku-86 protein levels were used as loading controls. (c) Relative E2, E3, E4, and L3 RNA in infected HBTECs at the indicated times p.i., as assayed by qRT-PCR. HBTECs were infected with the dl312, wt and dl312, and DM viruses at the same MOIs as those described for panel a. Values are plotted relative to those of wt and dl312 at 18 h p.i. Data are represented as averages ± standard deviations (SD) from three independent experiments.