FIG 2.

bub1 deficiency does not show enhanced Dcr2/RNAi and JAK-STAT antiviral signaling activity. (A and C) vago (A) and vir-1 (C) mRNAs expressed in the whole body, measured by qRT-PCR at 3 days postinfection. The change of expression was normalized to that of w¹¹¹⁸ flies at day 0. (B) GFP fluorescence intensity in S2* cells cotransfected with pAGW and gfp dsRNA in the presence or absence of bub1 dsRNA. All data were normalized to those for S2* cells transfected with only pAGW. All error bars represent SE of data from at least three independent tests (n > 15 flies [A and C] for each time point). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant (as determined by two-way ANOVA [A and C] or one-way ANOVA [B]).