FIG 5.

Bub1 facilitates endocytosis-dependent pathogen infection. (A) bub1-deficient flies are resistant to Listeria monocytogenes septic infection compared to wild-type (w¹¹¹⁸) flies. (B) Total CFU of Listeria monocytogenes from the whole body of the indicated flies at the indicated times. (C) Intracellular CFU of Listeria monocytogenes from the whole body of the indicated flies at the indicated times. Gentamicin was injected into the fly anterior abdomen 3 h before sample collection to eliminate extracellular bacteria. (D) VSV RNA levels in the whole body of the indicated flies were measured by qRT-PCR at the indicated times and normalized to that in w¹¹¹⁸ flies. (E) bub1 knockdown in S2* cells impairs VSV-GFP entry ability at 15 min and 30 min postinfection. VSV RNA was quantified by qRT-PCR. The percent VSV entry ability was calculated as mentioned above. All error bars represent SE of data from at least three independent tests (n > 60 flies [A] for each line or n > 20 flies [B to D] for each time point). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant (as determined by a Kaplan-Meier test [A], Student's t test [D and E], or two-way ANOVA [B and C]).