FIG 5.

The antiviral activity of eqMx2 exhibits substrate selectivity. A HEK293/eqMx2 cell line (eqMx2) constitutively expressing HA-tagged eqMx2 was constructed. A HEK293/GFP cell line (GFP) was used as the negative control. (A) Immunoblot analysis of HA-tagged eqMx2 protein. Staining for actin served as a loading control. (B to F) Both eqMx2 and GFP cell lines were inoculated with increasing amounts (10, 20, 50, and 100 μl) of one-life-cycle MLV (B), HIV-1 (NL4-3) (C), EIAV (D), SIVagm (E), and SIVmac (F) luciferase reporter viruses. Cells were lysed, and luciferase activity in the cell lysates was measured at 24 hpi. Mean relative infection efficiencies relative to GFP cell lines with standard deviations from three independent experiments are shown.