FIG 5.

25HC restricts reovirus infection as early as 2 h posttreatment. HeLa cells were treated with the ethanol vehicle control or 10 μM 25HC at the indicated times prior to infection. Cells were infected with T1L at an MOI of 10 PFU/cell and were fixed at 24 h postinfection, stained with anti-T1L antisera and DAPI, and analyzed by fluorescence microscopy. Error bars indicate 95% CI of the results of biological replicates. *, P < 0.05 by Student's t test. Data are representative of two independent experiments.