FIG 8.

25HC does not affect transferrin uptake. HeLa cells were treated with the ethanol vehicle control or 10 μM 25HC for 12 h. Cells were serum starved for 30 min at 37°C and pulse-labeled with Alexa 594-labeled human transferrin for 10 min, washed, and incubated in serum-containing medium for the indicated times, at which point the cells were fixed, stained with DAPI, and imaged by confocal microscopy.