FIG 2.

Relative positions and orientations of the PCR primers used in this study. The different combinations of primers used in nested or seminested PCRs are represented above the herpesviral DNA polymerase gene sequence, in different gray boxes. Primers XxxR1 and XxxR2, represented by dotted arrows, correspond to the different antisense specific primers used in a degenerate (CMV2F1 or CMV2F2)-nondegenerate nPCR assay (Table 2). Bars below the sequence represent the different nested PCR products expected. The pairs of primers on the left side of the bars indicate those used for the first-round PCR, while those on the right side correspond to those used in the nested PCRs. The sequences of the oligonucleotide primers are given in Table 2.