FIG 1.

E1/E2 gene amplification and inoculation. (A) Forward and reverse primers were designed to anneal at conserved sites flanking the E1 and E2 genes. (B) H1 and H2 were inoculated with aliquots of the same plasma containing unsequenced hepacivirus A strain WSU quasispecies. Two weeks postinfection, H1 plasma and H2 plasma were pooled, and aliquots were used to inoculate H3, SCID1, SCID2, foal 1, and foal 2. (C) Horses 753, 755, and 757 were inoculated with serum containing hepacivirus A strain CU quasispecies.