FIG 3.

EBNA3C and cyclin D2 colocalize in nuclear compartments. (A) HEK293T, Saos-2, and U2OS cells were cotransfected with Flag-tagged cyclin D2 and DsRed-tagged EBNA3C. The transfected cells were collected after 48 h of transfection, fixed, and incubated with mouse anti-Flag (M2) antibody and Alex Fluor 488-conjugated secondary antibody. DsRed-tagged EBNA3C was directly detected by fluorescence microscope. (B) BJAB, BJAB7, and LCL1 cells were semi-air dried and kept on slides. Endogenous EBNA3C (E3C) or cyclin D2 was incubated with its specific antibody, followed by the corresponding secondary antibodies. Nuclei were stained with DAPI. The images were captured with an Olympus FluoView FV300 microscope and analyzed using its FluoView software.