FIG 4.

ANCHOR3-GFP in AdV genome replication in fixed cells. Cells were mock infected or infected with Ad5-ΔE1ΔE3-ANCHOR3-GFP in the absence (top [long] row) or presence (middle and bottom [long] rows) of replicative Ad5-ΔE, as indicated on the top and left. At 24 hpi, cells displaying GFP fluorescence (green signal) were fixed and stained with DBP-specific antibodies (red signal). The long middle row shows cells with early RC and the long bottom row cells with late RC as defined previously (32). The two short rows at the bottom show enlargements of the boxed region in the last column depicting replicated DNA in early (I) and late (II) RC, respectively.