FIG 2.

Induction and spread of αS pathology in the M83^+/− mouse model following injection with mouse αS fibs. Mice were injected unilaterally in the sciatic nerve with mouse WT αS fibs (mfib αS) and harvested at 1 month (a) or 2 months (b) p.i. or when the mice reached the end stage of disease (c). (d) Mice were also injected with human αS fibs containing the E46K mutation (hfib αS E46K) and harvested at the end stage of disease or, for those with no symptoms, at 12 months p.i. Representative images show αS pathology in both the ipsilateral (ipsi-) and contralateral (contra-) DRG and several CNS regions, depicted by cartoons with black dots representing the specific locations the images were taken (n ≥ 8 animals per cohort). Tissue sections were stained with an antibody to αS phosphorylated at Ser129 (2G12) and counterstained with hematoxylin. Scale bars = 50 μm. Sp.C., spinal cord.