FIG 6.

Accumulation of gliosis in M83^+/− injected mice. M83^+/− mice were injected unilaterally in the sciatic nerve with control inocula (PBS and WT SOD1 fibs) and harvested at 6 months p.i. or with mouse WT αS fibs (mfib αS) and harvested at 1 month or 2 months p.i. or when the mice reached the end stage of disease. (a) Spinal cords were immunostained to visualize astrocytes using an antibody to GFAP and representative images were captured (n = 3 animals per cohort). (b) GFAP immunoreactivity in the cervical and lumbar segments of the spinal cord was quantified for each cohort (n = 3 animals per cohort; mean ± SD). ***, P ≤ 0.001. (c) Spinal cords were also immunostained for microglia using an antibody to cd11b, and representative images were captured (n = 3 animals per cohort). Scale bars = 100 μm.