FIG 4.

The host genes into which the lentiviral vector integrated did not influence HCV replication. (a) Huh7.5-1 and no. 18 cells were transfected with the full-length version or an N-terminal (the first 3 exons before the lentiviral vector integration site) fragment of ZNF517, after which they were infected with HCV at an MOI of 2 24 h after transfection. The protein levels of ZNF517 and HCV core were assayed by Western blotting 48 h after infection. (b) Huh7.5-1 and no. 30 cells were transfected with the full-length or an N-terminal (the first 9 exons before the lentiviral vector integration site) fragment of EMSY, after which they were infected with HCV at an MOI of 2 24 h after transfection. The protein levels of EMSY and HCV core were measured by Western blotting 48 h after infection.