TABLE 1.
Summarized results of inverse PCR
| Cell clone | Lentiviral vector integration site |
Inhibition of HCV | Inhibition of eGFP | |||
|---|---|---|---|---|---|---|
| Chromosome | Gene symbol or GenBank accession no. | Sequencea | Direction of integration | |||
| 13 | 19 | MYO9B (2nd intron) | AAACCCCGTC67290TCTACTAAAA | Forward | Yes | Yes |
| 14 | 17 | SMURF2P1b | ACCATTATTG3313TTTTTGAAAA | Reverse | No | No |
| 17 | 1 | AC006500.4c | TCTTAAGTAG4061GGGACCTTCA | Reverse | No | No |
| 18 | 8 | ZNF517 (3rd intron) | CAGAGTCTTC6177TCTAACTACC | Forward | Yes | No |
| 22 | 12 | SARNP (9th intron) | CTGTAGTCCC39477AACTACTCGG | Forward | Yes | Yes |
| 23 | 17 | AC091529.1c | AACTCACTAG134359CTCAAGATCA | Forward | No | No |
| 30 | 11 | EMSY (9th intron) | GCTTTGTCAC54075CCAGGCTGGA | Forward | Yes | Yes |
a
The nucleotides after which the lentiviral vector integrated are in boldface. The numbers indicate the positions of the nucleotides in the sequences.
b
SMURF2P1 is a pseudogene.
c
The genomic fragment does not contain genes.