FIG 7.

The putative replication compartment stains for the polymerase-associated processivity factor pUL44, while pp28 and gB proteins accumulate in a region close to the nucleus suggestive of the assembly compartment. (a and b) TB40-ANCHOR3 HCMV-infected MRC5 cells were stained for pUL44 at different times p.i. (a) Twenty-four hours p.i. (b) At 72 h p.i., only the putative RC was positive for pUL44, confirming its status. Upper left, Hoechst 33342; upper right, OR-GFP fluorescence; lower left, anti-pUL44; lower right, merge. (c to e) TB40-ANCHOR3 HCMV-infected MRC5 cells were stained for the pp28 tegument protein at different times p.i. (c and d) or for the envelope gB protein (e). (c) Twenty-four hours p.i., pp28 was already expressed but appeared diffuse in the whole cell. (d) Seventy-two hours p.i., pp28 was concentrated in a large region close to the nucleus where OR-GFP spots were also visible, suggesting the region is the assembly compartment. Upper left, Hoechst 33342; upper right, OR-GFP fluorescence; lower left, anti-pp28; lower right, merge. (e) TB40-ANCHOR3 HCMV-infected MRC5 cells were stained for the gB envelope protein 72 h p.i., showing accumulation in a region close to the nucleus, especially in the central area of the region. Upper left, Hoechst 33342; upper right, OR-GFP fluorescence; lower left, anti-gB; lower right, merge. Scale bars, 5 μm.