FIG 8.

Visualization of infected cell by correlative fluorescence/electron microscopy. (a) Cells infected with TB40-ANCHOR3 HCMV were first analyzed by fluorescence microscopy 96 h p.i. (left) and then fixed and processed for electron microscopy (EM) examination (right). The zone boxed in yellow was further analyzed at higher magnification, as shown in panel d. (b) In the RC, electron microscopy revealed different forms of capsids resembling the typical type A, B, and C capsids (black, red, and white arrows, respectively). (c) At higher magnification, other, possibly more diverse forms of capsids seem to appear, some containing dense fragmented material (arrows). (d) In the chosen area (boxed in yellow in panel a), 4 capsids were observed by EM, all of which correspond to fluorescent spots. Three appeared as B forms (white arrows), while the fourth was an A form (red arrow). Spots associated with the three type B capsids presented similarly weak intensities, suggesting they contained a single viral genome. On the other hand, the type A capsid coincided with a much brighter spot and could be associated with a replicating structure with several genomes.