TABLE 1.

Measurement of TB40-ANCHOR3 HCMV stock virus titers

Stock virus amt (μl)	Measured virus titera
	Plaque-forming assay		Fluorescence assay
	2-h infection	18-h infection	2-h infection	18-h infection
10−4	6.0 × 107	2.0 × 108
10−3	5.2 × 107	1.6 × 108	2.1 × 107	1.2 × 108
10−2	2.8 × 107		3.9 × 107	4.2 × 108
10−1			1.2 × 108	1.9 × 108

^aTiters were measured using a classical plaque-forming assay and a fluorescence assay. See Materials and Methods for details. The measured titers were significantly higher when infection time was increased from 2 h to 18 h. Similar results were obtained with both techniques.