FIG 2.

NbHDA6 localization and expression. (A) Subcellular localization of HDA6. Micrographs showing cells expressing NbHDA6-GFP, AtHDA6-GFP, or GFP were examined under GFP fluorescence (left panels), RFP fluorescence (middle panels), or an overlay of GFP/RFP/bright illumination (right panels) by confocal microscopy. RFP-H2B was used as a marker for the nucleus. Bars, 20 μm. (B) Western blot analysis to detect NbHDA6-GFP, AtHDA6-GFP, or GFP protein expression using anti-GFP antibody. The gel was stained with Ponceau S to show protein loadings. (C) Real-time RT-PCR analysis of NbHDA6 mRNA levels in N. benthamiana. GAPDH was used as an internal control. Each data set was derived from at least three biological repeats.