FIG 9.

Characterization of the naive CD4⁺ T cell reservoir in ART-treated SIV-infected RM infants. (A) Flow-cytometric assessment of CCR5, PD-1, Ki67, and HLA-DR expression at the surfaces of peripheral bulk, naive, SCM, CM/TM, and EM CD4⁺ T cells. (B) Flow-cytometric analyses of IFN-γ expression on splenic naive, SCM, CM/TM, and EM CD4⁺ T cells following ex vivo stimulation with PMA/ionomycin. *, P < 0.05. (C) Assessment of the frequencies of naive and memory CD4⁺ T cells carrying replication-competent SIV in ART-suppressed RM infants by quantitative viral outgrowth assay.