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. 2018 Aug 29;92(18):e01035-18. doi: 10.1128/JVI.01035-18

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FIG 3 — Effects of mutation(s) in UL51 on the steady-state expression levels of UL51. (A) HaCaT cells were mock infected (lane 1) or infected with wild-type HSV-1(F) (lane 2), YK631 (UL51-S184A) (lane 3), YK632 (UL51-S184D) (lane 4), YK633 (UL51-S184A/D-repair) (lane 5), YK634 (UL51-T190A) (lane 6), YK635 (UL51-T190A-repair) (lane 7), YK636 (UL51-S226T228S235/AAA) (lane 8), or YK637 (UL51-S226T228S235/AAA-repair) (lane 9) at an MOI of 5, harvested at 18 h postinfection, and analyzed by immunoblotting with antibodies to UL51, ICP8, and β-actin. (B) HaCaT cells were mock infected (lane 1) or infected with wild-type HSV-1(F) (lane 2), YK638 (ΔUL51) (lane 3), or YK639 (ΔUL51-repair) (lane 4) at an MOI of 5 and analyzed as described for panel A. A molecular mass marker is indicated on the left.