FIG 9.

Effects of mutation(s) in UL51 on the localization of UL31 and UL34 in HaCaT cells. (A) HaCaT cells were infected with wild-type HSV-1(F), YK631 (UL51-S184A), YK632 (UL51-S184D), YK633 (UL51-S184A/D-repair), YK634 (UL51-T190A), YK636 (UL51-S226T228S235/AAA), YK638 (ΔUL51), or YK639 (ΔUL51-repair) at an MOI of 5, fixed at 18 h postinfection, permeabilized, stained with anti-UL34 and anti-UL31 antibodies, and examined by confocal microscopy. Each image in the far-right column is the magnified image of the boxed area in the image to its left. Because different patterns of UL31 and UL34 localization were observed in cells infected with each of the indicated viruses, images of two of the infected cells are shown here as examples of UL31 and UL34 localization. (B) Quantitation of infected HaCaT cells with aberrant punctate structures of UL31 and UL34 adjacent to the nuclear rim. The experiments were performed as described for panel A, and the percentage of cells with aberrant punctate structures at the nuclear rim was determined. Each value is the mean ± standard error of the results from three independent experiments. ***, P < 0.001 (by Tukey's test); n.s., not significant.