T cell responses in individual vaccinated monkeys. PBMCs were stimulated with CBD1, K24W, K27W, and HIV-Gag p24 peptides. IL-2 (A), IFN-γ (B), and IL-4 (C) ELISpot assays were performed at different time points. Data are expressed as spot-forming cells (SFC) per million PBMCs minus the background (medium alone), which did not exceed 80 SFC per million cells. Arrowheads indicate immunizations.