TABLE 4.
IgG titers before and after the SHIV challenge in macaques immunized with a CBD1/CBM cocktaila
| Macaque | Time (wk) before or after challenge | IgG titer |
||||
|---|---|---|---|---|---|---|
| Cocktail | CBD1 | K27W | K24W | Gag298–312 | ||
| 30164 | −2 | 32,000 | 4,000 | 16,000 | 16,000 | 1,000 |
| 30164 | +3 | 16,000 | 4,000 | 8,000 | 8,000 | 1,000 |
| 30164 | +8 | 16,000 | 4,000 | 8,000 | 16,000 | 1,500 |
| 30237 | −2 | 64,000 | 3,000 | 20,000 | 32,000 | 1,000 |
| 30237 | +3 | 64,000 | 3,000 | 16,000 | 32,000 | 1,000 |
| 30237 | +8 | 64,000 | 2,000 | 16,000 | 32,000 | 1,000 |
| 30317 | −2 | 16,000 | 4,000 | 16,000 | 16,000 | 2,000 |
| 30317 | +3 | 12,000 | 4,000 | 8,000 | 16,000 | 2,000 |
| 30317 | +8 | 8,000 | 2,000 | 8,000 | 16,000 | 2,000 |
| 30788 | −2 | 32,000 | 2,000 | 16,000 | 8,000 | 1,500 |
| 30788 | +3 | 16,000 | 2,000 | 16,000 | 4,000 | 1,000 |
| 30788 | +8 | 16,000 | 2,000 | 16,000 | 8,000 | 1,000 |
| 30809 | −2 | 16,000 | 32,000 | 16,000 | 6,000 | 1,500 |
| 30809 | +3 | 16,000 | 30,000 | 16,000 | 6,000 | 2,000 |
| 30809 | +8 | 16,000 | 30,000 | 8,000 | 6,000 | 1,500 |
a
Cynomolgus macaques were challenged weekly by repeated exposure to low doses of SHIV. The titers of IgG, 2 weeks before (−2) and after (weeks +3 and +8) SHIV challenges, were assessed by ELISA against the cocktail peptides used for immunization as well as individual peptides. The titers correspond to the dilution of antipeptide antibodies giving an OD value of ≥0.1 in ELISA.