FIG 1.

Six MCMV vTFs form a complex. HEK293T cells were cotransfected with the six MCMV vTFs (pM49, pM79, pM87, pM91, pM92, and pM95) with the indicated tags and collected at 48 h posttransfection. HCMV protein pUL38 was used as a negative control. Cell lysates were subjected to immunoprecipitation (IP) using anti-FLAG M2 (mouse monoclonal) magnetic beads, which capture FLAG-tagged pM87 (A), FLAG-tagged pM95 (B), FLAG-tagged pM79 (C), FLAG-tagged pM91 (D), FLAG-tagged pM49 (E), and FLAG-tagged pM92 (F). The whole-cell lysates (INPUT) and the immunoprecipitated fractions were immunoblotted (IB) with the indicated antibodies to detect their interactions.