Fig. 1.

Competitive inhibition of Gal-3 binding to ASF by multivalent glyconanomaterials 24–39 as determined by ELISA. The following sample dose response inhibition curves are shown. a Inhibition by monovalent disaccharides lactose, LacNAc and LacdiNAc, by glyconanomaterials 30, 39 (two best inhibitors in the series), and 34 (the worst inhibitor in the series). The sigmoidal curves yield the values of IC₅₀ for the respective compounds. b Inhibition by glyconanomaterials 30, 31, and 32 (structural motif LacdiNAc-N-triazole). Here, the concentrations are calculated for the active substance of the glycomaterial—LacdiNAc glycan—and thus the curves yield the values of IC₅₀ per glycan. The higher the density of glycans on the polymer backbone, the lower the inhibitory potency per glycan. c Inhibition by glyconanomaterials 24, 29, 34 and 35 (containing various linkers with a comparable molar content of LacdiNAc). Here again, the curves yield the values of IC₅₀ per glycan. The type of attachment to the polymer backbone predestined the inhibitory potency per glycan