Table 1.
Characteristics of polymer precursors used for the conjugation of LacdiNAc epitope
| Compound | Prepared from | Precursor for glycopolymer | Functional groups | Mn (g/mol)a | Ð a | |
|---|---|---|---|---|---|---|
| Type | Content (mol. %) | |||||
| 10 | 8, 9 | – | TT | 11.4 | 22,900 | 1.06 |
| 11 | 8, 9 | – | TT | 20.5 | 25,300 | 1.13 |
| 12 | 8, 9 | – | TT | 31.0 | 27,700 | 1.04 |
| 13 | 10 | 24, 25, 29, 30 | Propargyl | 10.3 | 21,400 | 1.07 |
| 14 | 11 | 26, 31 | Propargyl | 20.1 | 22,700 | 1.14 |
| 15 | 12 | 27, 28, 32 | Propargyl | 29.4 | 20,700 | 1.18 |
| 16 | 10 | 33, 34 | DBCO | 5.4 | 21,500 | 1.22 |
| 17 | 10 | 35 | Ethynylphenyl | 9.9 | 21,200 | 1.07 |
| 18 | 11 | 36, 37 | Ethynylphenyl | 20.2 | 27,400 | 1.11 |
| 20 | 8, 19 | – | NH2 | 8.5 | 27,500 | 1.08 |
| 23 | 20 | 38, 39 | Diethynylphenyl | 6.8b | 31,700 | 1.11 |
aMolecular weight (Mn) and dispersity (Ð) of polymer precursors were determined using GPC with RI and MALS detection using TSKgel Super SW3000 column and methanol/0.3 M sodium acetate buffer, pH 6.5 (4/1, v/v) as the mobile phase
b6.8 mol. % is the content of linker molecule corresponding to 13.6 mol. % of ethynylphenyl moieties available for CuAAC