Table 2.
Effect of 8-week HFD on enzymatic and nonenzymatic antioxidants, total antioxidant/oxidant status, and oxidative damage products in the rat's plasma and serum.
| C (n = 8) | HFD (n = 8) | |
|---|---|---|
| GPx (mU/mg protein) | 0.9 ± 0.09 | 1.6 ± 0.1∗ |
| GR (nU/mg protein) | 55.3 ± 0.7 | 56.55 ± 0.2 |
| CAT (nmol H2O2/min/mg protein) | 3.9 ± 0.2 | 6.2 ± 0.2∗ |
| SOD-1 (mU/mg protein) | 29.4 ± 1.3 | 42.9 ± 1.6∗ |
| UA (μg/mg protein) | 3.4 ± 0.3 | 4.90 ± 0.3∗ |
| TAC (Trolox nmol/mg protein) | 9.6 ± 0.2 | 13.0 ± 0.9∗ |
| TOS (nmol H2O2 equiv./mg protein) | 36.9 ± 1.9 | 126.0 ± 5.0∗ |
| OSI | 433.5 ± 32.7 | 976.0 ± 38.2∗ |
| FRAP (nmol/mg protein) | 1.15 ± 0.3 | 1.91 ± 0.2∗ |
| AGE (AFU/mg protein) | 29.7 ± 0.6 | 83.6 ± 0.2∗ |
| 4-HNE (fg/mg protein) | 51.5 ± 5.5 | 170.8 ± 10.6∗ |
| MDA (ng/mg protein) | 70.2 ± 8.2 | 267.4 ± 15.5∗ |
| 8-OHdG (pg/mg protein) | 6.6 ± 0.3 | 8.6 ± 0.3∗ |
4-HNE, 4-hydroxynonenal protein adducts; 8-OHdG, 8-hydroxy-2′-deoxyguanosine; AGE, advanced glycation end products; CAT, catalase; FRAP, ferric reducing ability of sample; GPx, glutathione peroxidase; GR, glutathione reductase; HFD, high fat diet; MDA, malondialdehyde; OSI, oxidative stress index; SOD-1, Cu-Zn-superoxide dismutase-1; TAC, total antioxidant capacity; TOS, total oxidant status; UA, uric acid. Enzymatic antioxidants (GPx, GR, CAT, and SOD-1) were determined in serum whereas other markers were assayed in plasma. Differences statistically important at ∗p < 0.05.