TABLE 1.
Classa | Markers | Frequency of cell population in mouse group |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
BALB/c |
BALB/c latency |
P vs BALB/cb | FKO |
Latency × FKO |
P vs FKOb | Latency × FKO × TLR4mt |
P vs latency × FKOb | |||||||
% | SD | % | SD | % | SD | % | SD | % | SD | |||||
Mature | CD19+ IgM+ IgD+ | 33 | 3.2 | 30 | 4.3 | NS | 36 | 2.0 | 35 | 4.2 | NS | 39 | 3.5 | NS |
Activated | CD19+ IgM+ IgD+ FSChic | 6.2 | 0.4 | 6.4 | 1.1 | NS | 9.1 | 1.3 | 8.9 | 0.9 | NS | 9.8 | 1.1 | NS |
MZ | CD19+ IgM+ IgD− | 4.3 | 0.6 | 5.1 | 1.9 | NS | 6.1 | 1.2 | 5.5 | 1.6 | NS | 5.7 | 1.1 | NS |
FO | CD19+ CD21− CD23+ | 39 | 3.3 | 35 | 5.5 | NS | 39 | 3.2 | 40 | 3.6 | NS | 44 | 4.8 | NS |
Spleen | ||||||||||||||
PBs | CD19− B220+ CD138+ | 0.06 | 0.02 | 0.13 | 0.07 | 0.04 | 0.19 | 0.05 | 0.19 | 0.04 | NS | 0.21 | 0.06 | NS |
PCs | CD19− B220− CD138+ | 0.20 | 0.06 | 0.37 | 0.08 | 0.001 | 0.39 | 0.10 | 0.77 | 0.20 | 0.002 | 0.50 | 0.13 | 0.018 |
BM | ||||||||||||||
PBs | CD19− B220+ CD138+ | 0.28 | 0.02 | 0.42 | 0.14 | 0.04 | 0.40 | 0.07 | 0.37 | 0.10 | NS | 0.53 | 0.04 | 0.004 |
PCs | CD19− B220− CD138+ | 0.45 | 0.008 | 0.50 | 0.11 | NS | 0.44 | 0.10 | 0.58 | 0.20 | NS | 0.55 | 0.13 | NS |
MZ, marginal zone B cells; FO, follicular B cells; PBs, plasmablasts; PCs, plasma cells; BM, bone marrow.
Data were analyzed using Student's t test. n = 6 for all genotypes. NS, not significant.
FSChi, high forward scatter.