FIG 1.

Passage of VSV-MAK-GP leads to emergence of glycoprotein mutations in HuH7 and Vero cells. The illustration shows the experimental approach for passage of WT VSV-MAK-GP on Vero E6 and HuH7 cells. Five replicates were analyzed for Vero cells at passage 1 (sequencing results are summarized in the boxes on the right). Five replicates were analyzed for HuH7 cells at passage 1 (plate P1) and at passage 2 (plate P2).