FIG 3.
HCMV NAb induction by MVAB-7Ag1 and control vectors in C57BL/6 mice. C57BL/6 mice (n = 4 or 5) were immunized three times in 4-week intervals by the intraperitoneal (i.p.) route with MVAB-7Ag1 or the control vectors (MVAB-PC/gB, MVAB-PC/pp65, MVAB-PC, MVAB-gB, or MVAB-pp65) and HCMV immune responses were evaluated 1 week postimmunization. MVA-pp65/IE and MVA-Venus were used as additional controls. NAb titers (NT50; geometric mean titer) were measured in mouse immune sera on ARPE-19 or MRC-5 cells against HCMV strain TB40/E (A and C), TR (B and D), Towne (E), or AD169 (F) in the absence (−) or presence (+) of 5% guinea pig complement (abbreviated C). Dotted lines indicate the lowest serum dilution analyzed. Bars represent geometric means with 95% confidence intervals. Statistical significance of differences between NAb titers were calculated using two-way ANOVA, followed by Sidak's multiple-comparison test.