TABLE 1.
Primer pairs for recombination
| Primer pair (5′–3′)a | Insertion siteb | Genome positionb |
|---|---|---|
| GAATATGACTAAACCGATGACCATTTAAAAACCCCTCTCTAGCTTTCACTAAAAATTGAAAATAAATACAAAGGTTC | 44L/45L | 37331 |
| ATAATGTTTTTATATTATACATGTTCTAAAAGAATAATCGATACAGTTTACTAGTATAAAAAGGCGCGCC | ||
| AATTGTACTTTGTAATATAATGATATATATTTTCACTTTATCTCATTTGATTTTTATAAAAATTGAAAATAAATACAAAGGTTC | 64L/65L | 56742 |
| ATTCCGAAATCTGTACATCATGCAGTGGTTAAACAAAAACATTTTTATTCCTAGTATAAAAAGGCGCGCC | ||
| ATATGAATATGATTTCAGATACTATATTTGTTCCTGTAGATAATAACTAAAAATTTTTATCTAGTATAAAAAGGCGCGCC | 69R/70L | 63812 |
| GGAAAATTTTTCATCTCTAAAAAAAGATGTGGTCATTAGAGTTTGATTTTTATAAAAATTGAAAATAAATACAAAGGTTC | ||
| ATTGATAATATAAATATGAGCATTAGTATTTCTGTGGATTAATAGATTTTTATAAAAATTGAAAATAAATACAAAGGTTC | 148R/149L | 137504 |
| TTATGAGGTATTTAGAGATTAGAGATGATTAATGATCCCCATACTAGAAATTTTTATCTAGTATAAAAAGGCGCGCC | ||
| TTGGGGAAATATGAACCTGACATGATTAAGATTGCTCTTTCGGTGGCTGGTAAAAAATTGAAAATAAATACAAAGGTTC | Del3 | 149342 |
| ACAAAATTATGTATTTTGTTCTATCAACTACCTATAAAACTTTCCAAATACTAGTATAAAAAGGCGCGCC | ||
| GGAAAGAATCTACTCATCTAAACGATTTAGTAAACTTGACTAAATCTTAATTTTTATAAAAATTGAAAATAAATACAAAGGTTC | Del3 res. (163R/167R) | 148381- 151019 |
| TAAATTTTAAGTTTTACGTGGTAAGTTTTAATATTTAACTAATACATTAGCTAGTATAAAAAGGCGCGCC |
a
Underlined sequences were used to mediate recombination.
b
Insertion sites and genome positions based on GenBank accession no. U94848.