FIG 4.

Requirement of the NLRP3-binding ability of V protein for inhibition of the NLRP3 inflammasome. (A) Schematic diagram of the SeV V mutants Vn (aa 1 to 317), Vc (aa 318 to 384), and Vcys (C362S and C365R). (B) HEK293T cells were transfected with plasmids encoding NLRP3 inflammasome signaling molecules together with the indicated plasmid. Immunoprecipitation and immunoblot analysis were performed as described for Fig. 3A. Data are representative of those from three independent experiments. (C) HEK293T cells were transfected with plasmids encoding NLRP3 inflammasome signaling molecules together with the indicated plasmid. IL-1β levels in cell supernatants were determined as described for Fig. 2A. Data were derived from at least three or four independent experiments and are presented as mean values. SD are shown as error bars. ***, P < 0.001.