FIG 5.
Effect of SeV V protein on NLRP3 inflammasome assembly. (A and B) HEK293T cells in an 8-well microscope glass slide with a removable 8-well chamber were transfected with plasmids encoding NLRP3-GFP and ASC-Flag, together with the indicated plasmid encoding VSeV-V5His. (A) Subcellular localizations of NLRP3-GFP (green), ASC-Flag (red), VSeV-VH (blue-green), and nuclei (DAPI; blue) were visualized by confocal microscopy. The scale bars represent 10 μm. (B) ASC speck-forming cells and VSeV-positive cells were quantified from five randomly selected fields, and the percentages of ASC speck- and VSeV-positive cells in total VSeV-positive cells are shown. Data were derived from at least three or four independent experiments and are represented as mean values. SD are shown as error bars. **, P < 0.01. (C) Experimental data on BRET signals at 24 h posttransfection were derived from three independent experiments and are represented as mean values. SD are shown as error bars. **, P < 0.01 versus transfection with empty vector.