FIG 1.

Structure of Nup153 FG in complex with hexameric HIV-1 capsid. (A) Structure of NUP153 FG peptide (green) in its binding pocket within the hexameric HIV-1 capsid. The capsid side chains that contact the peptide are shown as sticks and labeled. N57 is binding Nup153 (B), PF74 (C) (42), and BI2 (D) (43). Hydrogen bonds are shown as dashed yellow lines. (E) Production and maturation of HIV-1 capsid mutants viruses. The indicated HIV-1 viruses were produced in human 293T cells. Producer cells and purified viruses were analyzed for HIV-1 capsid expression by Western blotting using anti-p24 antibodies. Producer cells validated for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression were used as loading controls. Experiments were performed three times, and a representative example is shown.