FIG 1.

AP-1(A/S) activates expression of BMRF1 (EA-D), an EBV marker early protein, but does not induce expression of BFRF3, an EBV marker late protein, or DNA replication. (A) Western blot of BZKO cells harvested 48 h after transfection with control CMV plasmid vector, wild-type ZEBRA, Z(S186A), wild-type AP-1, or mutant AP-1(A/S), probed with the antibodies indicated. (B) qPCR for the expression of viral DNA performed on the same cells used for Western blot analysis in panel A. Western blotting and qPCR were performed once on cells used for the array shown in Fig. 3.