FIG 10.

Both ZEBRA and AP-1(A/S) bind to the BDLF3 promoter. (A) Schematic drawing of the locus of BDLF1 to BDLF3 showing the locations of the six primer sets used for ChIP qPCR, indicated by 1 to 6, and the probe used for Northern blotting. The TATA box is used to denote the start of transcripts, and the poly(A) signal is used to denote the end of transcripts. The human herpesvirus 4 complete wild-type genome (GenBank accession number AJ507799.2) sequence was used to demarcate locations of genes. ORF, open reading frame. (B) Chromatin immunoprecipitation was performed in BZKO cells harvested 48 h after transfection with control FLAG vector, wild-type FLAG-ZEBRA, or mutant FLAG-AP-1(A/S). The FLAG antibody was used to immunoprecipitate all target proteins. Association to BDLF3 was determined using qPCR. Bar height indicates the mean of three experiments. Error bars show one standard error of the mean. P values were determined using pairwise Student's t tests with Dunnett's correction for multiple comparisons compared to results with the control FLAG vector. *, P ≤ 0.0332; **, P ≤ 0.0021.