FIG 12.

Induction of Raji cells with TPA leads to expression of viral late RNAs in the absence of viral DNA replication. (A) Fold change in gene expression in Raji cells treated with TPA relative to levels in untreated cells at 48 h. Each point indicates one replicate. The horizontal lines denote the means. (B) Fold change in expression of viral DNA measured by qPCR, relative to levels in uninduced cells, was performed on the same cells used for RT-qPCR analysis. Each point indicates one replicate. The horizontal lines denote the means. (C) Western blotting was performed on Raji cells harvested 48 h after lytic induction with TPA. Lanes 1 to 3, biological replicates treated with TPA; lanes 4 to 6, untreated biological replicates; lane 7, positive control for FR3 in HH514-16 cells treated with sodium butyrate at 72 h as described in the legend of Fig. 11.