FIG 2.

AP-1(A/S) binds to oriLyt and to Rp of ZRE2 and -3. Chromatin immunoprecipitation was performed in BZKO cells harvested 48 h after transfection with a control vector, wild-type FLAG-ZEBRA, FLAG-Z(S186A), wild-type FLAG-AP-1, or mutant FLAG-AP-1(A/S). The FLAG antibody was used to immunoprecipitate all target proteins. Association to oriLyt (A) or Rp of ZRE2 and -3 (B) was determined using qPCR. Bar height indicates the mean of three experiments. Error bars show 95% confidence intervals determined using bootstrapping. ZEBRA, AP-1, and AP-1(A/S) all bind significantly to oriLyt and Rp, with no statistically significant differences among them.