FIG 7.

Timing of inhibitor addition to block entry of MERS-CoV into cells. (A) Dec-RVKR-CMK (100 μM), camostat (10 μM) plus E64d (10 μM), or lopinavir (30 μM) was added to LoVo cells (for 30 min on ice) at the indicated times before and after MERS-CoV inoculation. The amount of viral mRNA in cells at 6 h postinfection was measured by real-time PCR (n = 1). (B) Calu-3 cells were used instead of LoVo cells; all experiments were carried out as described for panel A (excluding lopinavir treatment) (n = 1).