FIG 1.

ADCML of SIV and SIV-infected cells. (A and B) Sera collected from all 60 macaques at week 57 were diluted as indicated and analyzed for ADCML of SIV. The average percent input SIV lysis is shown per dilution per animal cohort. The keys identify the animal cohorts immunized with gp120 or gp140, and error bars represent SEM. F, female; M, male. (C) Individual SIV ADCML assay well contents from 3 vaccinated macaques were analyzed for complement protein C3 activation via Western blotting; a representative blot from a single animal is shown. Row A represents C3 (185 kDa), and row B represents the C3 cleavage product C3b (110 kDa). +, components within each SIV ADCML assay well analyzed. (D) Western blot C3b band/C3 band density ratios were averaged per lane from 3 individual Western blots and are displayed in the histogram representing heat-inactivated serum only (lane 1 in panel C), nonspecific activity (Act.) (lane 3 in panel C), and SIV-specific activity (lane 4 in panel C). Error bars represent SEM. (E) Sera collected from 30 vaccinated macaques at week 57 were diluted 1:50 and analyzed for ADCML of SIV in the presence or absence of SPS. The symbols represent values from individual macaques. (F and G) Sera collected from all 60 macaques at week 57 were diluted as indicated and analyzed for ADCML of SIV-infected H9 cells. The average percent input SIV-infected cell lysis is shown per dilution per animal cohort. The keys identify the animal cohorts, and error bars represent SEM. (H and I) The ADCML of SIV and SIV-infected H9 cells was compared for all 60 animals. SIV and SIV-infected H9 cell lysis values are displayed per animal as two dots connected by a line. The keys identify the animal cohorts. Asterisks indicate significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001). Abbreviations: HiS and HiSerum, heat-inactivated serum; CompS, complement serum; CVF, cobra venom factor.