FIG 5.
Regulation of global and template-specific translation by PKCβ activity. (A and B) Specific inhibition of PKCβ with LY333531 reduces TPA-inducible translation of Cox-2, uPAR, HIF1-α, MKP-2, and p21Cip1 mRNAs (A) and global protein synthesis (B). HeLa cells were Dox induced (72 h), serum starved (24 h), pretreated with 3 nM LY333531 (2 h), TPA stimulated (up to 5 h), and analyzed as shown in Fig. 4. (C to E) HeLa cells were serum starved (24 h), pretreated with 3 nM LY333531 (2 h), and TPA stimulated (5 h) (C). Tandem samples were used for RNA isolation, RT-qPCR, and immunoblotting and analyzed as described for Fig. 4D and E (D and E).