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Comparison of the observed mass with masses calculated for combinations of linked candidate proteins.
| Protein conjugate | Theoretical mass |
|---|---|
| (MH+, monoisotopic, Da) | |
| Rub1-UbD77 | 17345.38 |
| Rub1T72R-Rub1 | 17299.49 |
| Rub1T72R-UbD77 | 17286.39 |
| Rub1-Ub | 17230.35 |
| Rub1T72R-Ub | 17171.36 |
| Ub-Ub | 17102.22 |
| Rub1T72R-Rub1T72R | 17240.50 |
| Observed Mass (MH+, monoisotopic): 17286.42 Da | |
