Figure 2:

Growth curves of P. gingivalis W83 showing the antibacterial activities of anaerobically prepared nanoparticles. Bacterial growth over a 24-hour period was assessed by measuring the absorbance of cultures at 600 nm for specific intervals. P. gingivalis W83 was inoculated with anaerobically prepared (≈0.3 nM, 500 μL) glutathione capped Ag and Ag/Au ((I) Au: Ag = 0.10 ± 0.04; (II) Au: Ag = 0.30 ± 0.05; (III) Au: Ag = 2.2 ± 0.1) nanoparticles in 5 mL total volume. Glutathione capping was done using method II. Error bars represent the standard deviation of three experiments.